Photo-closslinkable chitosan: KytoGEL



The clinical demands on KytoGEL

"Wound care based on physical adhesion"

• Ordinary blood products include the coagulant factors of mammalian protein origin. Therefore these are difficult to use to the patients involving vascular disorder or taking anti-blood coagulants.

“Development goal for NeTech”

• is to supply bio-degradable wound dressing that is physically adhesive and can be used in all sorts of situations.

The anxiety of bacterial and fungal infection risks is eliminated from polysaccharides chitosan. Thereby chitosan is promising as a degradable material for wound care.

Applicable materials for wound dressing
Mammalian proteins	Chemicals	Carbohydrates
Fibrinogen	Cyanoacrylate	Starch
Fibrin	Polyethylene glycol	Cellulose
Thrombin	Polyurethane	Gum Arabic
Collagen	Superabsorbent polymer	Sodium Alginate
Gelatin		chitosan
Albumin
Negative factors
Viral infection	Low degradability	Low adhesiveness
High cost	Toxicity	Purification
	Low elasticity

Characteristic of the chitosan

Advantages	Biodegradable
	Biocompatible
	Antibacterial
Weaknesses	Aqueous chitosan: strong acidity
	Difficulty for endotoxin removal

KytoGEL which does not involve chitosan’s weaknesses is a photoreactive polysaccharide.

• Diminished endotoxin (reverse osmosis water level)
• Neutral liquid (pH 6～7)
• Hydrogelation and adhesion arising from irradiating ultraviolet-A

• We can supply KytoGEL liquid having stabilities of medicine level.

The operation of KytoGEL

Specification

Bulk powder	> Shrimp Chitosan (deacetylation: over 90 %) > White powder of KyteGEL does not smell or gives an slight alcohol smell.  > The powder of KytoGEL is difficult to dissolve in water and hardly dissolves     in ethanol. > The powder absorbs water, swells up, and then becomes viscous liquid.
Physical properties	> KytoGEL liquid converts itself into hydrogel through irradiating UV-A within     390 nm. > KytoGEL covalently reacts  to injured  tissue, thereby its hydrogel strongly     adheres than fibrin glue. (Journal of Biomedical Materials Research     Volume 49(2), 289–295, 2000) > Hydrogel made by KytoGEL is viscous than fibrin glue. > Autoclavable > Stable at room temperature for over one year

Antibacterial	> Growth inhibition assay to Escherichia coli, staphylococcus aureus,     Pseudomonas aeruginosa, hay bacillus and Klebsiella pneumoniae     indicates the property of antibacterial that is equal to versatile     methyl paraben. > Antivirus function develops through the addition of silver-based     antibacterial.
Safety data	> Endotoxin: Less than 0.2 EU/mg powder*      (ordinary use: 1~2 wt% liquid). > Cytotoxicity: negative* > Reverse mutation: negative* > No serological abnormality (rats)      *Entrusted to Hadano research center institute,        food and drug safety center
Formulation	> NeTech supplies the raw materials which guaranteed viscosity,     photo-reactivity, pH, and stability.
Animal studies	> KytoGEL is degradable within a month into subcutaneous tissue     (mouse/rat) and submucosa (pig). > The validity as hemostat, tissue adhesive, wound healing, topical DDS     and the like  was proved.  > There are also many clinical study concerning chitosan and a chitosan     derivative.       “Chitosan preparations for wounds and burns: antimicrobial and        wound healing effects” Tianhong Dai, et al. Expert Rev Anti Infect        Ther.  2011; 9(7): 857–879.

Hemostat
Topical DDS
Endoscope
Wound care