Pioneer of the chemically functionalized glucides
NeTech Inc.

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[Basic functions of lectin]
Comparison of the cellular affinities against lectins





Difference of cellular affinity against SBA-lectin

  • Materials and methods
  • 丸31.jpgG-Coat dishes modified with α-1,6-galactose
  • 丸31.jpgSBA (Soybean agglutinin) is a plantal glycoprotein composed of four subunits having
  • BIBa specific binding domain to N-acetylgalactosamine and galactose.
  • 丸31.jpgVenous blood of healthy adults was used to collect blood cells except hematopoietic BIBstem cells collecting from cord blood.
  • 丸31.jpgIdentification of blood cell types was carried out by flow cytometry using monoclonal BIBantibodies (Beckton Dickinson).

LBEI std.jpg

  • Results
  • 丸31.jpgThe order of cell adhesion based on the affinity with lectins:
  • BIBErythroid, granulocytes > lymphocytes >NK celis
  • 丸31.jpgIn the case of applying less than 100 μg/mL of SBA, erythrocytes preferentially BIBadhered to the dishes. Thereby leukocytes were isolated from erythrocytes as
  • BIBan unattached cell fraction.
  • 丸31.jpgThe adhesion of the immature stem cells was 21% even if the dishes were treated BIBby over 800 μg/mL of SBA.




Enrichment of NK cells using ECL-lectin (Negative selection)

  • Materials and methods
  • 丸31.jpgThe dishes (Φ: 35mm) were modified with G-coat involving galactose and glucose, BIBand then treated with galacto-specific ECL (10 ng/mL) at room for 60 min.
  • 丸31.jpgPeripheral blood mononuclear cells (PBMCs) were enriched by 1.077 g/mL single-BIBdensity gradient centrifugation.
  • 丸31.jpgPBMCs (2x103-6.jpg) including NK cells were resuspended in dPBS(-) with 0.2 wt% of BIBBSA.
  • 丸31.jpgThe suspended cells were seeded on the ECL-treated dishes, and then incubated at BIB37℃ for 30 min.
  • 丸31.jpgNonadherent cells in supernatant were recovered from the dishes, and then NK cells BIBand lymphocytes in the recovery were identified by Flow cytometry using
  • BIBmonoclonal antibodies.

NK.jpg

  • Results
  • 丸31.jpgThe loss of NK cells was suppressed by diminishing galactose ratio on the dishes, BIBto lead to more selective collection of NK cells.
  • 丸31.jpgThe adjustment of buffer or incubation conditions may improve the efficiency of NK BIBcell enrichment.




LinkIconPrenatal test
LinkIconEfficacy of lectin
LinkIconPracticability
LinkIconRipple effect